In health and disease, cells do not exist in isolation but form communities of specialized cells that interact and shape each other’s fate. These cellular neighborhoods are made up of common building blocks, but how these building blocks interact differs across disease states. In this webinar, we will demonstrate how single-cell spatial phenotyping with PhenoCycler™ (formerly CODEX®) provides a unique insight into cellular neighborhoods. We will discuss the cellular neighborhoods uncovered in a recent study from Garry Nolan’s lab at Stanford University and provide a walk-through of the bioinformatic tools available to reveal complex cellular neighborhoods in single-cell spatial phenotyping datasets.