Developing and Validating a High-throughput 9-color Immunofluorescence Assay for Translational Studies in Immuno-oncology Research

We have developed and optimized a 9-color assay and validated its reproducibility across two imaging platforms — the Mantra Workstation and the Vectra Polaris (now rebranded as the PhenoImager HT). In this poster, we demonstrate a translational workflow which offers an optimal balance between multiplexing and sample throughput to facilitate research and support translational studies on whole FFPE tissue.