Analysis of FFPE Human Tumor Tissues Using PhenoCycler (formerly CODEX) with Signal Amplification

We expanded our CODEX (now rebranded as PhenoCycler) workflow to amplify the fluorescent signal intensities of low expressing biomarkers by combining Tyramide Signal Amplification (TSA) with CODEX, also known as CODEX® Amp. In this poster, we present the analysis of CODEX data for a panel of over 20 biomarkers on human FFPE tissue, including signal amplification of PD-L1, PD-1 and FOXP3.