Your Learning Hub for Multiplexed Imaging

We’ve rebranded some of our products, learn more ›

CODEX® is now PhenoCycler, Phenoptics™ is now Phenolmager.

Akoya Academy

Virtual Training On PhenoCycler and PhenoImager Workflows

We’re committed to connecting with you through Akoya Academy virtual training sessions to share tips, tricks, and best practices on using our products. We’ll continue updating the schedule, so bookmark this page to track the latest additions.

PhenoCycler 2-part Webinar Series

June 20 and June 27, 2023
PhenoCycler Fusion forward

PhenoImager HT 2-part Webinar Series

June 22 and June 26, 2023
PhenoImager HT Spatial Signatures

On Demand Webinars

Available to watch now

Introducing PhenoImager HT 2.0: Onboard Spectral Unmixing for a Ready-to-Analyze mIF Workflow

Ocotober 10, 2023
Speaker:
  • Carla Coltharp, PhD | Associate Director, Systems Integration, Akoya Biosciences
  • Aditya Pratapa, PhD Sr. Data Scientist, Akoya Biosciences
You Will Learn:
  • Learn about the feature upgrades with HT 2.0 including onboard spectral unmixing
  • Learn how to set up an imaging and unmixing protocol with HT 2.0
  • Understand the new HT 2.0 data analysis workflow

PhenoCycler Analysis Solutions: Exploring the Akoya Analysis Ecosystem

June 27, 2023

Speaker:

  • Anne Kwei | Director of Product Management, Akoya Biosciences
  • Aditya Pratapa, PhD Applications Scientist, Akoya Biosciences

You Will Learn:

  • Learn how Akoya’s ecosystem of software partners can be tailored to fit your analysis needs
  • Explore the various options for seamless data integration from PhenoCycler-Fusion images to actionable datasets
  • The exciting innovations and capabilities of various software solutions that can supercharge your analysis for more insightful data

Developing Reproducible Multiplex Imaging Workflows for the PhenoImager Platform

June 26, 2023

Speakers:

  • Kristen Legendre | Technical Application Scientist, Akoya Biosciences
  • Ladan Gheiratmand, PhD | Field Application Scientist, Akoya Biosciences

You Will Learn:

  • Learn about the PhenoImager solution for rapid, accurate spatial phenotyping.
  • Hear best practices for multiplex immunofluorescence (mIF) assay development using Opal for high sensitivity staining.
  • Find out how PhenoCode Signature assays simplify protein spatial phenotyping and can accelerate spatial signature development. 
PhenoImager Data Analysis Using QuPath and Python
June 22, 2023

Speakers:

  • Aditya Pratapa, PhD Applications Scientist, Akoya Biosciences
  • Ning Ma, PhD | Applications Scientist, Akoya Biosciences

You Will Learn:

  • Gain a comprehensive understanding of the PhenoImager data analysis workflow
  • Explore open-source data analysis pipeline using QuPath and Python
  • Enhance your ability to interpret complex spatial phenotypes and extract valuable information such as SpatialScore
PhenoCycler-Fusion: High-parameter Single-cell Spatial Biology
June 20, 2023

Speaker:

  • Clemens Duerrschmid, PhD | Sr. Technical Application Scientist, Akoya Biosciences

You Will Learn:

  • Learn about the power of comprehensive spatial phenotyping and its applications
  • Understand how PhenoCycler-Fusion functions as part of an integrated end-to-end solution for spatial phenotyping
  • Discover how high-quality whole-slide images open the door to endless possibilities for your research

PhenoImager Solutions: Bringing Speed and Scale to the Forefront of Multiplex Imaging and Spatial Phenotyping

April 28, 2022 | 8:00 AM PST | 11:00 AM EST | 5:00 PM CEST

Speakers:

  • Amélie Viratham, MS | Reagents Field Application Scientist, EMEA, Akoya Biosciences

You Will Learn:

  • How the PhenoImager Fusion enables the development of more relevant and highly predictive biomarkers, called spatial phenotypic signatures 
  • How Multispectral Imaging (MSI) technology leads to quantitative and accurate phenotyping 
  • How our universal antibody chemistry promotes a seamless continuum from Discovery to Translational Research

Understanding Spatial Phenotyping: A Webinar Series by Akoya Academy

August 24 – September 7, 2021

Speakers:

  • Clemens Duerrschmid, Ph.D. | Technical Application Scientist,Akoya Biosciences
  • Grady Carlson, Ph.D. | Bioinformatics Field Application Scientist, Akoya Biosciences
  • Leilani Marty-Santos, Ph.D. | Field Application Scientist, Akoya Biosciences

You Will Learn:

  • How to better understand complex spatial phenotypes using phenoptrReports
  • Getting even more information from your spatial data including charts, tables, network maps and images
  • How to set up inForm® to get the most out of both inForm® and phenoptrReports

PhenoptrReports: Unlocking the Power of Spatial Biology Analysis

August 19, 2021 | 8:00 AM PST | 11:00 AM EST | 5:00 PM CEST
Speakers:
  • Ivan Masetto | Field Application Scientist, Phenoptics & CODEX, Akoya Biosciences, Inc.
You Will Learn:
  • How to better understand complex spatial phenotypes using phenoptrReports
  • Getting even more information from your spatial data including charts, tables, network maps and images
  • How to set up inForm to get the most out of both inForm and phenoptrReports

Spatial Phenotyping with Opal from Manual to Automated Multiplex Staining

July 20, 2021 | 7:00 AM PST | 10:00 AM EST | 4:00 PM CEST
Speakers:
  • Virginie Goubert | Strategy & Business Development Manager, Europe, Akoya Biosciences, Inc.
You Will Learn:
  • The steps to automating your manual workflow
  • Guidelines for determining optimal Opal dye dilutions
  • Pitfalls to avoid during development of your automated assay

R&D Sneak Peek | Opal Multiplex and Simulated H&E on The Same Tissue Section

April 20, 2021 | 8:00 AM PST | 11:00 AM EST | 5:00 PM CET
Speakers:
  • Linden Wyatt, Ph.D. | Technical Application Scientist, Northeast US, Akoya Biosciences, Inc.
  • Michael McLane, BS | Associate Reagents Scientist, Akoya Biosciences, Inc.
You Will Learn:
  • Not all H&E is created equal. See how simulated H&E allows for superior morphological context
  • The benefits of viewing H&E and multiplex stains on the same tissue.
  • How to make the most out of your valuable tissue sections—conduct mIF and H&E on the same slide without using serial sections or tissue alignment software
  • How Opal™ staining complements standard H&E analysis with a more quantitative and precise methodology.

How Autofluorescence Reduction can Improve Your CODEX Workflow

March 2, 2021 | 8:00 AM PST |11:00 AM EST | 5:00 PM CET

Speakers:

  • Oliver Braubach, Ph.D. | Senior Manager Applications, Akoya Biosciences, Inc.
  • Nadya Nikulina | Senior Application Scientist, Akoya Biosciences, Inc.
  • Bassem Ben Cheikh, Ph.D. | Data Scientist, Akoya Biosciences, Inc.
  • Cassandra Hempel | Research Associate II, Akoya Biosciences, Inc.

You Will Learn:

  • How to set up autofluorescence quenching in the PhenoCycler workflow
  • The effect of autofluorescence reduction on PhenoCycler data and workflow
  • Analytical metrics that will assess the effectiveness and safety of this protocol

How Opal Multiplex Staining can Accelerate Neuroscience Research

October 26, 2020 | 1:00 PM China ST | 1:00 AM EDT | 5:00 PM GMT
Speaker:
  • Dr. Ke-qi Fan | Research Lead, Jin Jin Molecular Immunology Lab, Life Sciences Institute of Zhejiang University
You Will Learn:
  • How multiplex technology has been leveraged in neuroscience
  • How Dr. Fan successfully characterized an immune response linked to anxiety-like behavior
  • Gain a better understanding of stress-induced metabolic disorder

Instant Gratification: Multiplex IF in a Snap with Mantra™

Recorded on August 25, 2020
Speaker:
  • Clemens Duerrschmid, Ph.D. | Technical Application Scientist, Akoya Biosciences, Inc.
You Will Learn:
  • Warp speed assay development
  • Seamless workflow for scale up to high throughput deployment
  • Parallel path for digital pathology
  • Multitasking for multiplex IF

Just Add Tissue | The Ready-to–Use Phenoptics Workflow

Recorded on July 14, 2020

Speakers:

  • Najiba Mammadova, Ph.D. | Reagent Sales Specialist, Akoya Biosciences, Inc.
  • Agnes Haggerty, Ph.D. | Technical Applications Scientist, Akoya Biosciences, Inc.

You Will Learn:

  • Fully automated 6-Plex, 7 color on the BOND RX
  • Streamlined Vectra® Polaris™ imaging
  • Threshold based phenotyping for lung cancer and melanoma
  • See easy phenotyping in action with an inForm® demo

Autofluorescence | What Lies Beneath?

Recorded on June 16, 2020
Speaker:
  • Roslyn Lloyd | Sr. Application Scientist, PhenoImager & PhenoCycler, Akoya Biosciences, Inc.
Agenda:
  • Making it easy with Bias-free multispectral analysis
  • See how top research labs are unleashing the full power of spatial biology
  • Proper Autofluorescence isolation: the impact of revealing hidden biomarkers to enable quantitative pathology

Tips & Tricks to Optimize PhenoCycler Images

Recorded on May 19, 2020

Speakers:

  • Mahdis Rahmani | Sr. Field Application Scientist, Akoya Biosciences

Agenda:

  • Tips on how to optimize PhenoCycler images for use in presentations and scientific literature
  • Live Q&A

Analyzing Cell Suspensions with PhenoCycler MAV

Recorded on May 12, 2020

Speakers:

  • Oliver Braubach, Ph.D. | Senior Applications Scientist, Akoya Biosciences

Agenda:

  • How to do single-cell analysis of cell suspensions with the PhenoCycler workflow
  • A walkthrough and demo of the MAV gating function using PBMCs as an example
  • Live Q&A

Best Practices for Developing Custom PhenoCycler Panels

Recorded on Apr 28, 2020

Speakers:

  • Meenu Perera, Ph.D. | Sr. Field Application Scientist, Akoya Biosciences

Agenda:

  • Recommendations for custom conjugation and validation
  • Factors to consider during PhenoCycler panel design – a customer case example
  • Live Q&A

A Deep Dive on the Multiplex Analysis Viewer (MAV) to Analyze PhenoCycler Images

Recorded on Apr 21, 2020
Speakers:
  • Grady Carlson, Ph.D. | Bioinformatics Field Applications Scientist, Akoya Biosciences
Agenda:
  • An introduction to MAV and its features– gating, unsupervised clustering, dimensionality reduction plots (e.g., tSNE), and spatial interaction plots and tables
  • A walkthrough and demo of MAV with a case example
  • Live Q&A