Tips & Tricks Archives

Analyzing 2.5 Million Cells with PhenoCycler-Fusion: Explore the Dataset

Collection: Tips & Tricks
Tags: Spatial Analysis

From the early stages of planning to research and development to execution, Akoya is extremely excited to roll out the first data captured by the PhenoCycler-Fusion platform. Since launch, PhenoCycler-Fusion has generated lots of excitement, setting a new standard in spatial biology for high-speed…

The Importance of Antibody Validation

Collection: Tips & Tricks
Tags: Immunology

One of the most critical steps in multiplexed antibody panel design is selecting the right antibodies for your study. Choose the wrong antibody and you may lose weeks if not months, worth of work. An rigorous antibody validation strategy keeps your program on track by: Proving antibody specificity (the antibody’s ability to differentiate between…

3 Things You Should Know About Breast Tissue Organoids

Collection: Tips & Tricks
Tags: Cancer

Organoids are tissue-derived adult stem cells that can be grow into self-organizing organotypic structures. Due to their derivation from human tissue, organoids can more accurately model the interactions between healthy human tissue and cancer, enabling patient-specific drug testing and development of tailored therapeutic regimens. The…

Spatial Analysis with Seurat: Unlocking Single-Cell Insights from CODEX Data

Collection: Tips & Tricks
Tags: Spatial Analysis

Seurat is an R package developed by Rahul Satija’s lab at the New York Genome Center. A widely used, open-source tool for single-cell analysis, Seurat was designed to explore single-cell RNA sequencing data. Seurat can also be applied to multiplex imaging-based spatial phenotyping data generated with CODEX®. Bioinformatics Field Application Scientist,…

Spatial Analysis of Cellular Neighborhoods: Uncovering Complex Interactions in Tissue Biology

Collection: Publication Reviews, Tips & Tricks
Tags: Spatial Analysis

The tumor microenvironment is undeniably complex. Specialized immune cells interact directly and indirectly with tumor cells in a constantly evolving relationship that influences the biology of the tumor. Spatial context is essential to uncovering the mysteries of cell behavior and interactions. Just as individuals may behave differently in cities, suburbs,…

Show Off Your Creativity by Participating in the Akoya Image Contest: Four Examples from Our Team

Collection: Tips & Tricks

Our social media image contest is in full swing! We’re inviting all our followers to get creative and show off your interests with tissue images. Here’s how you can enter. Either produce a tissue image using one of Akoya’s platforms or find one on our website. Then, put your creative skills to use and edit the image to show off a fun activity or theme. Share…

Spatial Analysis Tools to Explore the Tissue Microenvironment: inForm Tissue Analysis

Collection: Tips & Tricks
Tags: Spatial Analysis

As researchers attempt to characterize cellular activity within the tissue microenvironment, it’s critical for them to have the tools they need to extract meaningful biological information from tissues. This is essential for basic research and drug development studies, as…

Spatial Analysis Tools to Explore the Tissue Microenvironment: The CODEX® Multiplex Analysis Viewer

Collection: Tips & Tricks
Tags: Spatial Analysis

With the right analysis tools, researchers can move from multiplexed imaging data to visualizing the spatially resolved cellular structure and cellular phenotypes of the tissue microenvironment. As part of the CODEX® solution for spatially resolved, multiplexed im…

Your Questions, Answered – A Deep Dive on the CODEX MAV

Collection: Tips & Tricks
Tags: Spatial Analysis

In our recent Akoya Academy webinar, “A Deep Dive on the Multiplex Analysis Viewer (MAV) to Analyze CODEX Images”, we heard from Grady Carlson, PhD, Technical…

CODEX Software Suite Unlocks the Power of Single-Cell, Spatial Analysis in FFPE Tissue

Collection: Tips & Tricks
Tags: Spatial Analysis

Single-cell RNA-sequencing methods have become a mainstay tool for cancer researchers. Techniques like CITE-Seq can analyze the expression patterns of large sets of mRNAs and proteins in hundreds…

CODEX® is now PhenoCycler, Phenoptics™ is now Phenolmager.

Category: Tips & Tricks