PhenoCycler-Fusion Assays
Scalable and Flexible Deep Spatial Phenotyping
We’ve rebranded some of our products, learn more ›
CODEX® is now PhenoCycler, Phenoptics™ is now Phenolmager.
Unlock Deep Spatial Discoveries with PhenoCycler-Fusion 2.0 Assays
No matter the scale of your experiment—be it a 20-plex study, a 40-plex exploration, or a deep 100-plex spatial phenotyping assay—PhenoCycler®-Fusion 2.0 Assays can seamlessly adapt to the needs of your spatial discoveries. Powered by proprietary barcoded antibody technology and leveraging an extensive database of over 350 antibodies, PhenoCycler®-Fusion 2.0 Assays make deep spatial phenotyping easier than ever.
Choose from ready-to-use PhenoCode™ Discovery Panels for time-efficient studies or design your own panels to suit your specific research needs. With the PhenoCyler-Fusion 2.0 system and PhenoCycler®-Fusion 2.0 Assays, you can embark on a spatial journey where scalability meets unparalleled flexibility, transforming the pace of your spatial biology research.
What Sets PhenoCycler-Fusion Assays Apart?
Scalable Plexing
Our barcoded antibody technology enables deep spatial phenotyping, combining antibody specificity with molecular barcodes to simultaneously detect 100+ targets at high spatial resolution, preserving tissue integrity.
Unmatched Content
Explore our extensive database of 350+ antibodies, covering 100+ phenotypes, and 30+ tissue types across multiple species.
Unparalleled Flexibility
Enjoy the convenience of ready-to-use PhenoCode Discovery Panels for time-efficient studies, or design your own panel.
How does Barcoding Technology Work?
The multiplexing capability of the PhenoCycler-Fusion 2.0 system is underpinned by the fundamental principles of barcoded antibody technology. In this framework:
- Each PhenoCycler antibody is intricately linked to a unique oligonucleotide sequence, known as the PhenoCycler Barcode.
- Each PhenoCycler Reporter is a fluorescent dye molecule tagged with a short, complementary oligonucleotide sequence designed specifically for the associated PhenoCycler antibody.
- When the barcoded antibody and the reporter with the complementary oligo are incubated they undergo hybridization. This results in the generation of a fluorescent signal that can be effectively captured for each biomarker of interest.
Unlock Your Next IO Breakthrough with the IO60 Panel
The Fastest Ultrahigh-Plex Panel for IO Research
Curated from insights across 1,500+ publications and expertly designed to navigate the complexities of the IO landscape, the PhenoCode IO60 panel is the fastest ultrahigh-plex panel for IO research. When paired with the cutting-edge PhenoCycler®-Fusion 2.0, you can analyze 60 markers simultaneously and process over 16 samples per week.
- Comprehensive
- Fastest
- Scalable
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IO60 Spatial Omics Grant
Push the boundaries of single-cell and spatial transcriptomics with advanced spatial proteomics on the PhenoCycler®-Fusion 2.0 with our IO Spatial Omics Grant. The winning researcher will receive four samples processed with the 60-marker PhenoCode IO60 panel, creating a comprehensive spatial proteomics dataset, tailored to enhance your single-cell and spatial transcriptomics work with Akoya’s expert support.
Want to Design Your Own Panels?
Explore Akoya's extensive antibody database, a dynamic and ever-growing resource designed to build your own panels.
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Ready to Order PhenoCycler-Fusion Reagents?
PhenoCode Discovery Panels and Antibodies
PhenoCode Discovery Panels
Product
Technical Spec
PhenoCode Discovery IO60 Human Protein Panel
Antibodies
PhenoCycler Antibodies For Human FFPE Tissue
PhenoCycler antibodies have been tested in multiple types of human FFPE tissue and are conjugated to a distinct PhenoCycler Barcode (BX000). Twenty-five (25) test scan be performed with each kit.
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Product
Size
Anti-Hu Bcl-2(AKYP0120)-BX085—Atto 550 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu Caveolin(AKYP0115)-BX086—Alexa Fluor™ 647 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu CD21(AKYP0061)-BX032—Alexa Fluor™ 750 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu CD31(AKYP0047)-BX001—Atto 550 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu CD34(AKYP0088)-BX025—Alexa Fluor™ 647 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu CD38(AKYP0110)-BX035—Alexa Fluor™ 750 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu CD45(AKYP0074)-BX021—Atto 550 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu CD45RO(AKYP0059)-BX017—Alexa Fluor™ 647 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu CD79a(AKYP0109)-BX090—Atto 550 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu CD107a(AKYP0004)-BX006—Alexa Fluor™ 750 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu Granzyme B(AKYP0086)-BX041—Alexa Fluor™ 647 for PhenoCycler (optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu Histone H3 Phospho (Ser28)(AKYP0060)-BX030—Alexa Fluor™ 647 for PhenoCycler
25 tests
Anti-Hu HLA-A(AKYP0078)-BX029—Atto 550 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu HLA-DR(AKYP0063)-BX033—Alexa Fluor™ 750 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu IDO1(AKYP0084)-BX027—Alexa Fluor™ 750 for PhenoCycler (Optimized for PhenoCycler)
25 tests
Anti-Hu/Mu Ki67(AKYP0052)-BX047—Alexa Fluor™ 750 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
Anti-Hu PCNA(AKYP0085)-BX036—Alexa Fluor™ 750 for PhenoCycler (Optimized for PhenoCycler-Fusion)
25 tests
PhenoCycler Inventoried Antibodies for Human Fresh Frozen Tissue
PhenoCycler antibodies for human fresh frozen tissue have been tested using tonsil tissue. These PhenoCycler antibodies are conjugated to a distinct PhenoCycler barcode (BX000). Each kit contains one tube of PhenoCycler antibody and one tube of the corresponding dye-labeled reporter (RX000), sufficient volume for 25 PhenoCycler runs (or tests) at the standard staining conditions. Recommended dyes for human fresh frozen tissue are Atto 550, Cy5 or Alexa Fluor™ 647, and Alexa Fluor™ 488.
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Product
Size
PhenoCycler Inventoried Antibodies for Mouse Fresh Frozen Tissue
PhenoCycler antibodies for mouse fresh frozen tissue have been tested using spleen tissue and are conjugated to a distinct PhenoCycler barcode (BX000). Twenty-five (25) tests can be performed with each kit.
Catalog #
Product
Size
Anti-Hu/Mu CD49f(AKYP0018)-BX033—Alexa Fluor™ 647 for PhenoCycler (Formerly CD49f-BX033(GoH3)—Alexa Fluor™ 647-RX033)
25 tests
PhenoCycler Kits and Reagents
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Size
PhenoCycler Barcodes
Each Kit contains one tube of the lyophilized barcode and two tubes of reporters enough to perform between 10 to 50 stains depending on yield and titer
PhenoCycler Barcodes with Alexa Fluor™ 488 Reporters
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PhenoCycler Barcodes with Alexa Fluor™ 647 Reporters
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PhenoCycler Barcodes with Alexa Fluor™ 750 Reporters
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PhenoCycler Barcodes with Atto 550 Reporters
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PhenoCycler Multiple Reporter Barcode Kits
Each kit contains one tube of the lyophilized Barcode for use in a single conjugation reaction, and six tubes of reporters with 3 different fluorophores for use in optimizing PhenoCycler antibody titration and PhenoCycler runs.
FFPE Multi-Reporter Barcode Kits
FFPE Multi-Reporter Kits contain 2 vials each of Atto 550, Alexa Fluor™ 647, and Alexa Fluor™ 750 reporters. For each barcode, there is sufficient volume for 50 μg antibody conjugation, which provides enough material for 10 to 50 stains depending on yield and titer.
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Size
Fresh Frozen Multi-Reporter Barcode Kits
Fresh Frozen Multi-Reporter Kits contain 2 vials each of Alexa Fluor™ 488, Atto 550, and Alexa Fluor™ 647. For each barcode, there is sufficient volume for 50 μg antibody conjugation, which provides enough material for 10 to 50 stains depending on yield and titer.
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PhenoCycler Reporters
Individual Reporters can be ordered as needed. Each kit includes ample volume to accommodate 25 PhenoCycler-Fusion 2.0 Runs.
PhenoCycler Alexa Fluor™ 488 Reporters
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PhenoCycler Alexa Fluor™ 647 Reporters
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PhenoCycler Alexa Fluor™ 750 Reporters
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PhenoCycler Atto 550 Reporters
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