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Opal Polaris 7 Color IHC Detection Kits
Catalog Number: OP-000003
Opal™ Polaris Kits are part of a practical workflow for the simultaneous detection of up to eight tissue biomarkers plus nuclear counterstain within a single image, across the entire digital slide. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed. Opal Polaris 7-Color IHC Detection Kits are optimized for whole slide multispectral imaging with the Vectra® Polaris™ Automated Quantitative Pathology Imaging Instrument.
Catalog #
Product
Size
NEL861001KT
Opal 6-Plex Manual Detection Kit - for Whole Slide Imaging (formerly Opal Polaris 7 Color IHC Manual Detection Kit)
Up to 50 Slides
NEL871001KT
Opal 6-Plex Detection Kit - for Whole Slide Imaging (formerly Opal Polaris 7 Color IHC Automated Detection Kit)
Up to 50 Slides
Product Details
Specifications
Literature
Education Resources
Product Details
The Opal Polaris 7-Color Automation IHC Detection Kit is part of the Phenoptics 2.0 whole slide workflow and contains:
- 6 reactive fluorophores (Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780)
- Spectral DAPI
- 1X Plus Automation Amplification Diluent
- Blocking/Ab Diluent
- Opal Polymer HRP Ms + Rb
- Users should source primary antibodies and other workflow reagents separately. The Opal Polaris 7-Color Automation IHC Kit was optimized for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® Polaris™ quantitative pathology imaging system. Kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.
The Opal Polaris 7-Color Manual IHC Detection Kit is part of the Phenoptics 2.0 whole slide workflow and contains:
- 6 reactive fluorophores (Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780)
- Spectral DAPI
- 1X Plus Amplification Diluent
- Blocking/Ab Diluent
- Opal Polymer HRP Ms + Rb
- AR6 Buffer
- Users should source primary antibodies and other workflow reagents separately. The Opal 7-Color Manual IHC Kit was optimized for use on the Vectra® Polaris™ quantitative pathology imaging system. Kits contain enough reagents to stain 50-slides at the recommended 1:100 dilution ratio.
Specifications
Shipping Condition | Blue Ice |
Sample | Human |
Product Category | Opal Kits |
Type | Opal Detection Kit |
Detection Method | PhenoImager Fusion and PhenoImager HT |
Literature
Opal Storage and Stability
Storage, reconstitution and dilution of Opal dyes.
Technical Brief
BOND RX Protocol for MOTiF™ PD-1/PD-L1 Panel: Auto Melanoma Kit 50 Slides
Staining protocol
Education Resources