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1X Antibody Diluent / Block
This ready-to-use antibody blocking and diluent buffer formulation has been optimized specifically for Opal™ and TSA® Plus immunohistochemistry protocols.
Catalog #
Product
Size
Product Details
Specifications
Workflow
Literature
Education Resources
Product Details
This 2-in-1 antibody diluent for IHC and blocking buffer acts to minimize non-specific interaction of antibodies that can be a source of background
This formulation does not contain azide and should be compatible with all peroxidase based detection methods. Size: 100 mL.
- Ready to use formulation
- Convenient dropper bottle
- Azide-free, does not affect HRP
Specifications
Shipping Condition | Blue Ice |
Sample | Human and Mouse |
Product Category | Opal Kits |
Type | Opal Detection Kit |
Detection Method | Mantra 2, Vectra 3, PhenoImager Fusion, and PhenoImager HT |
Workflow
Bake and Deparaffinize Slides
- Bake at 60 C for one hour
- Xylene wash (3x ten minutes)
- Rehydrate with EtOH gradient into di H2O
Slide Fixation
- 10% NBF for 20 minutes
- Di H2O wash
Antigen Retrieval
- AR6 or AR9 MWT
- Cool to RT for at least 15 minutes
Blocking
- Di H2O rinse, TBST rinse
- PAP pen barrier
- Incubate tissue for 10 minutes in blocking solution at RT
Primary Antibody
- Remove blocker and add primary antibody
- Rinse with TBST, then wash 3 times for two minutes each in TBST
Secondary Antibody
- Incubation 10 minutes at RT
- Rinse with TBST and wash 3 times for 2 minutes each
Opal Reagent
- Opal fluorophore incubation for ten minutes at RT
- Rinse with TBST and wash 3 times for 2 minutes each
MWT
- Rinse slides with AR6 or AR9
- Place slides in microwave-safe jar, perform MWT
- Cool to RT for at least 15 minutes
Spectral DAPI
- Use AR6 for MWT prior to DAPI for removal of any unbound fluor
- Rinse slides in di H2O and then TBST
- Incubate DAPI for 5 minutes at RT
- Wash with di H2O and TBST two minutes each
Mount
- Apply monunting medium for fluorescene microscopy for coverslip
Literature
Education Resources