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Opal 3-Plex Detection Kits
formerly Opal 4-Color IHC kits
Opal™ 3-Plex Detection Kits provide a practical workflow for the simultaneous detection of up to 3 tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC is performed.
Product Details
Specifications
Literature
Education Resources
Product Details
The Opal 3-Plex Manual Detection Kit contains:
- 3 reactive fluorophores (Opal 520, Opal 570 and Opal 690)
- DMSO
- 10X Spectral DAPI
- 1X Plus Manual Amplification Diluent
- 1X Antibody Diluent/Block
- 1X Opal Anti-Ms + Rb HRP
- AR6 Buffer
The Opal 3-Plex Detection Kit contains:
- 3 reactive fluorophores (Opal 520, Opal 570 and Opal 690)
- DMSO
- 10X Spectral DAPI
- 1X Plus Automation Amplification Diluent
- 1X Antibody Diluent/Block
- 1X Opal Anti-Ms + Rb HRP
Users should source primary antibodies and other workflow reagents separately. The Opal 3-Plex Detection Kits were optimized for use on the Mantra 2, Vectra 3, PhenoImager Fusion and PhenoImager HT Systems. Kits contain enough reagents to stain up to 50-slides at the recommended 1:100 or 1:150 dilution ratio.
Specifications
Shipping Condition | Blue Ice |
Sample | Human |
Product Category | Opal Kits |
Type | Opal Detection Kit |
Detection Method | Mantra 2, Vectra 3, PhenoImager Fusion, and PhenoImager HT |
Literature
Opal Storage and Stability
Storage, reconstitution and dilution of Opal dyes.
Technical Brief
Education Resources