Observing cell-cell interactions is critical to understanding causative factors of biological outcomes. As the only benchtop platform capable of multiplexing >40 markers in tissue, CODEX® (rebranded to PhenoCycler™) uniquely enables these observations by assaying tissue biopsies using fluidics, an inverted microscope, oligo-conjugated antibodies, and fluorescent reporters. In this webinar, we will demonstrate CODEX single-cell spatial phenotyping using Seurat- one of the most widely used open-source platforms for single-cell RNA analysis. In addition, we will cover how to perform cellular neighborhood analysis adapted from Garry Nolan’s lab.