Rakuten Medical Inc., Principal Scientist, Chung-Wein Lee, PhD, describes the workflow outlined in his recently published manuscript in the Journal of Immunological Methods, titled Multiplex immunofluorescence staining and image analysis assay for diffuse large B cell lymphoma. Dr. Chung-Wein Lee and colleagues highlight that several multiplex immunofluorescence and image analysis assays have been described for solid tumors, but most are not adequate for lymphoma. In this video, Dr. Lee explains how his team used Akoya’s multispectral imaging platform and Opal staining kits to reliably interrogate the tumor microenvironment in large B-cell lymphoma, something that was previously a challenge.